Abstract
We performed a comparative study of the formation of H2?? foci (a marker of DNA doublestrand breaks) in human bone marrow mesenchymal stem cells after 24-h incubation with H-3-thimidin and tritium oxide with low specific activities (50-800 MBq/liter). The dependence of the number of H2AX foci on specific activity of 3H-thymidine was described by a linear equation y=2.21+43.45x (R-2=0.96), where y is the number of H2AX foci per nucleus and x is specific activity in 1000 MBq/liter. For tritium oxide, the relationship was described by a linear equation y=2.52+6.70x (R-2=0.97). Thus, the yield of DNA double-strand breaks after exposure to H-3-thymidine was 6.5-fold higher than after exposure to tritium oxide. Comparison of the effects of tritium oxide and X-ray radiation on the yield of DNA double-strand breaks showed that the relative biological efficiency of tritium oxide in a dose range of 3.78-60.26 mGy was 1.6-fold higher than that of X-ray radiation. Improvement of the methods of analysis of DNA double-strand breaks repair foci is highly promising in the context of creation of highly sensitive biodosimetry technologies for tritium compounds in humans.