Abstract
A new simple, accurate and reliable potentiometric method for determination of azithromycin was proposed and validated. Four types of sensors were fabricated. Conventional PVC plastic membrane (I), coated wire (II), carbon paste (III) and modified multi-wall carbon nanotubes carbon paste type (IV). The fabrication of all sensors based on the incorporation of azithromycin with phosphotungstic acid to form azithromycin-phosphotungstate as electroactive materials. The proposed sensors exhibit Nernstian response (55.10 +/- 0.19, 55.52 +/- 0.20, 57.09 +/- 0.14 and 58.04 +/- 0.11 mV decade(-1)) over concentration ranges 1.0 x 10(-6)-1.0 x 10(-2), 5.0 x 10(-7)-1.0 x 10(-2), 1.0 x 10(-7)-1.0 x 10(-2) and 1.0 x 10(-8)-1.0 x 10(-2) mol L-1 for sensor I, II, III and IV, respectively. The influence of several parameters such as pH, selectivity of sensors, response time and soaking time, etc. was studied. The proposed method was successfully applied for the determination of azithromycin in its pure form, pharmaceutical dosage forms and biological fluids. The obtained results were statistically analyzed and compared with those obtained by the reported method.