Abstract
This study investigated the enzymatic capacity of several P. anserina secretomes obtained via induction by various carbon sources. P. anserina secretomes displayed similar cellulase, xylanase and pectinase activity and greater arabinofuranosidase, arabinanase and galactanase activity than the levels found in T reesei. The secretomes were also tested for their capacity to supplement a T. reesei cocktail. Four of them improved the saccharification yield of steam exploded wheat straw by 14 to 48%. Our data strongly suggests that part of the improvement was due to complementary enzymes produced by P. anserina. Proteomic analysis of secretomes produced with Avicel and sugar beet pulp provided novel insights into the enzymatic arsenal of this fungus producing cellulases from families GH6 and 7 as well as producing GH10 xylanases, CE1 esterases, GH43 arabinofuranosidases and AA1 laccase-like multicopper oxidases. A striking point was the extensive production of lytic polysaccharide monooxygenases specifically in sugar beet-induced cultures.