Abstract
In the present study, iron oxide nanoparticles (Fe3O2-NPs) synthesized from ginger (Zingiber officinale) and cumin seeds (Cuminum Cyminum L.) extracts were investigated to reveal their potential to enhance the growth and drought resistance of wheat plants under drought stress. In an In Vitro experiment, four different concentrations for Fe3O2-NPs (0.3 mM, 0.6 mM, 0.9 mM, and 1.2 mM) of ginger and cumin seeds were tested. Among all the concentrations tested, ginger Fe3O2-NPs (0.6 mM) and cumin seeds Fe3O2-NPs (1.2 mM) were more effective to enhance wheat germination, biomass, and survival percentage under drought stress and irrigated conditions than the non-treated control plant. In a pot experiment, wheat plants under induced water stress showed marked up-regulation in the biochemical resistance mechanisms when treated with ginger Fe3O2-NPs (0.6 mM) and cumin seeds Fe3O2-NPs (1.2 mM) than the non-treated control. Cumin seeds Fe3O2-NPs (1.2 mM) were more effective than ginger Fe3O2-NPs (0.6 mM) in ameliorating adverse effects of drought stress in wheat. Results demonstrated that cumin seeds Fe3O2-NPs (1.2 mM) exhibited a higher increase in chlorophyll a, b and carotenoids (72%, 265% and 96% respectively), proline (127%), superoxide dismutase (115%), peroxidase (43.8%), ascorbate peroxidase (44.6%). This also showed higher reduction in lipid peroxidation, electrolyte leakage and increased soluble sugars and total Fe content in the roots and shoots than non-treated plants under drought. Hence, nano-priming can be considered an effective strategy for sustainable food production in marginal soils.
[Display omitted]
•Drought stress affects growth, photosynthesis and biochemical reactions in wheat.•Fe2O3-NPs synthesized from cumin and ginger ameliorates against drought stress.•The Fe2O3-NPs increases photosynthetic pigments, proline, reduced lipid peroxidation.•Cumin based Fe2O3-NPs showed excellent protection under drought stress than ginger.•This Fe2O3-NPs also showed improved antioxidative defense system in wheat plants.