Abstract
Comparative studies on the efficacy of methanolic extracts of (Punicagranatum) and triclabendazoal on Haemonchusspp by using scanning electron microscope (SEM) revealed that, Punicagranatumand ACBZ showed significant effect on adult worms of Heamonchuscontortousafter 24h in vitro cultivation, the worms started to stop motion animation and paralysis leading 100 % to death of worms.
Adult H. contortus: Scanning electron microscopy (SEM) of normal fresh worms showed, the mouth of the normal fresh worm was hexagonal with six semicircular rudimentary lips, lateral amphids, papillae and dorsal buccal lancet. The later was a specialized cuticular structure arising from the cuticular lining of the dorsal wall of the buccal cavity. The lancet's anterior tip and lateral edges were slightly rounded. A pair of cervical papillae was prominent and spine-like. The cuticle was transversally striated and with lateral ridges.
SEM of Treated WormsH. contortusafter 24 h incubation with 50 mg/ml ethanolic extracts of Punicagranatumpeelshowed the buccal capsule which presented a smooth surface in control worm, lost their normal aspect and showed distortion with severe blebbing of the lips. The cuticle including the lips appeared to be more swollen than normal The transverse striations became less pronounced and lost their normal aspect showing longitudinal wrinkles. The treated worms showed distortion of both buccal capsule and cuticle.
The lips were deformed and the cuticular surface had a wrinkled, corrugated appearance. Besides, longitudinal thickening and wrinkling of the cuticular ridges were observed.