Abstract
Development and validation of accurate and selective spectrophotometric methods were carried out for determination of a new combination of Mebendazole and Quinfamide in bulk powder and pharmaceutical formulation. Extended ratio subtraction coupled with isoabsorptive point methods were used for determination of the binary mixture. A comparative study was carried out between the newly developed methods; simultaneous ratio subtraction, absorbance subtraction, and dual wavelength spectrophotometric methods were applied as well. The developed methods were validated in accordance with the ICH guidelines. The developed methods were successfully carried out for determination of Quinfamide and Mebendazole in Vermox Plus® tablets. Upon statistical comparison of the results obtained by the developed methods with those obtained by the reported simultaneous equation spectrophotometric method, no significant difference was shown among them. Moreover, the introduced methods have the advantages of being more sensitive and have wider linearity ranges than other reported spectrophotometric methods.
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•Innovative, selective, eco-friendly spectrophotometric methods for determination of Mebendazole and Quinfamide•Extended ratio subtraction coupled with isoabsorptive point methods•Simultaneous ratio subtraction method•Absorbance subtraction method•Dual wavelength method•Comparative study among the developed methods