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Comparison of monoplex and duplex RT-PCR assays for the detection of measles virus
Journal article   Peer reviewed

Comparison of monoplex and duplex RT-PCR assays for the detection of measles virus

Khalifa Binkhamis, Hayley Gillis, Joseph Daniel Lafreniere, Joanne Hiebert, Lillian Mendoza, Janice Pettipas, Alberto Severini, Todd F. Hatchette and Jason J. LeBlanc
Journal of virological methods, Vol.239, pp.58-60
01/2017
PMID: 27838260

Abstract

Canada Hemagglutinin Measles Method Molecular Neuraminidase PCR
Rapid and accurate detection of measles virus is important for case diagnosis and public health management. This study compared the performance of two monoplex RT-PCR reactions targeting the H and N genes to a duplex RT-PCR targeting both genes simultaneously. The duplex simplified processing without compromising assay performance characteristic.

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