Abstract
In this study, we present the complete chloroplast genome of Dipterygium glaucum an important medicinal plant in Saudi Arabia, in order to evaluate the evolutionary relationship in the family Cleomaceae. The cp genome is second to be sequenced in Cleomaceae family, it has a circular structure and a length of 158,576 bp with 35.74% GC content. The genome is divided into four regions: LSC of 87,738 bp, SSC of 18,420 bp and a pair of inverted repeats 26,209 bp each. The chloroplast genome of D. glaucum encodes 116 different genes, including 81protien coding genes, 31tRNA genes and four rRNA genes. The phylogenetic relationship showed a close relationship between D. glaucum and Tarenaia hassleriana.