Sign in
Back
Cost-Effective Pooling of DNA from Nasopharyngeal Swab Samples for Large-Scale Detection of Bacteria by Real-Time PCR
Journal article   Peer reviewed

Cost-Effective Pooling of DNA from Nasopharyngeal Swab Samples for Large-Scale Detection of Bacteria by Real-Time PCR

Sophie Edouard, Elsa Prudent, Philippe Gautret, Ziad A. Memish and Didier Raoult
Journal of clinical microbiology, Vol.53(3), pp.1002-1004
01/03/2015
DOI: https://doi.org/10.1128/JCM.03609-14
PMCID: PMC4390636
PMID: 25552360

Abstract

Life Sciences & Biomedicine Microbiology Science & Technology
We investigated the potential of pooling DNA from nasopharyngeal specimens to reduce the cost of real-time PCR (RT-PCR) for bacterial detection. Lyophilization is required to reconcentrate DNA. This strategy yields a high specificity (86%) and a high sensitivity (96%). We estimate that compared to individual testing, 37% fewer RT-PCR tests are needed.

Metrics

1 Record Views

Details