Abstract
We described a simple, cost effective, non-toxic and environmental friendly green-chemistry reduction method to synthesized crystal nano silver (AgNPs) powder using CTAB and orange peel extract as reducing agent. The green reduced crystal nano silver powder was characterized using XRD analysis, Zeta sizer, Scanning Electron Microscopy (SEM) equipped with Energy Dispersive Spectroscopy (EDS) in addition to Transmission Electron Microscopy (TEM). The synthesized crystal nano silver showed promising antibacterial activity against gram negative (Escherichia coli) and gram positive (staphylococcus aureus and Streptococcus). The present study demonstrates the efficiency of synthesized nano silver as a cytotoxic agent against MCF-7 breast cancer cell line and HepG2 liver hepatocellular cancer cell line using by MTT assay. Thus, the synthesized crystal nano silver can be used as a wide range therapeutic agent against bacteria and cancer.