Abstract
The cytogenotoxicity of creatine monohydrate conc. (1.5, 2, 2.5 and 3g/100 ml) were examined on Vicia faba plant, i:e: meiotic division behavior, leaf protein by using SDS-PAGE protein electrophoresis and changes in DNA of M-2 faba bean plant by using RAPD analysis. All creatine monohydrate treatments showed abnormal pollen mother cells (PMCs) which increased as the concentration and treatment period increased. The most common abnormalities were stickines, disturbed, laggard, bridges and micro-multi nuclei. The electrophoretic study of leaf proteins showed alteration of some minor protein bands after creatine treatments. The highest concentration of creatine showed a polymorphic number of genetic bands by using RAPD-PCR product comparing with control. Results strongly suggested that creatine monohydrate is clastogenic. [Nora H. Al-zahrani, Kadija H. Alamoudi and Salha M. Al-shamrani. Cytogenetic and molecular variation on Vicia faba treated with creatine monohydrate. Life Sci J 2012;9(3):584-590] (ISSN: 1097-8135). http://www.lifesciencesite.com. 82