Abstract
Significant apoptotic effect in Hep2c cell line has been investigated, when diode laser (lambda = 635 nm, red) are used as a source of illumination and initiation of photodynamic action. The optimal uptake time of PhotofrinA (R) for Hep2c cell line was investigated by means of spectrophotometric measurement. Quantification of the live cell population was determined by means of neutral red assay (NRA). The spectrometry measurements showed that after 46 h incubation, the maximal cellular uptake of PhotofrinA (R) was achieved and photocytotoxic assay showed that light dose of 120 J/cm(2) give effective PDT outcome for Hep2c cell line incubated with 85 mu g/ml of PhotofrinA (R). No significant phototoxic and cytotoxic effects on Hep2c cells were observed due to light doses or photosensitize, when studied independently of each other and PhotofrinA (R) showed good anti tumor effects.