Abstract
Picea smithiana is an important tree, belonging to the family Pinaceae, having many medicinal uses. The local people use it for a variety of diseases including eye, kidney and skin. The plant was investigated scientifically for phytochemical constituents and cytotoxicity effect on human tumor cell line; MCF-7, K-562, HT-29, H -460, M-14 and HUTU-80. The results revealed the presence of alkaloids, sterols, carbohydrates, terpenoids, flavonoids, tannins, glycosides, phenols and saponins. The n-butanol fraction showed IC50 values below of 10 mu g/mL for MCF-7 and M-14 tumor cell lines, and above of 10 mu g/mL for 3T3 cells that was used as standard cell line to evidence cytotoxicity.