Abstract
Objective
To identify bladder cancer specific methylated DNA sequences for Saudi population in order to detect and predict bladder cancer progression.
Methods
In this study, we analyzed DNA methylation levels of 94 tumor suppressor genes loci in 24 bladder tissues (19 bladder cancer samples and 5 control samples taken from histologically normal bladders). DNA Methylation analysis was done using Human Tumor Suppressor Genes EpiTect Methyl II Complete PCR Array from Qiagen TM.
Results
We identified significant difference in DNA hypermethylation levels at APC, BRCA1, CDH1, CDH13, CDKN2A, DAPK1, ESR1, FHIT, MGMT, RASSF1, SOCS1, TIMP3, TP73, VHL, WIF1, E2F1, ERBB2, H1C1, OPCML, SFN, SFRP1, SFRP2, SPARC, and TERT between controls and cancerous samples. It was also observed that CADM1, DKK3 and SCGB3A1 were deferentially methylated in non‐muscle invasive versus muscle invasive bladder cancer samples. Additionally, DNA hypermethylation of ESR1 was notified as the novel tumor suppressor gene specific for Saudi population in bladder cancer.
Conclusion
Our findings suggest that these aberrant DNA methylation patterns in bladder cancer are disease and population specific and have a potential to develop as distinct DNA methylation based bio markers in future.
This is from the Experimental Biology 2018 Meeting. There is no full text article associated with this published in The FASEB Journal.