Abstract
In livestock, selection programs on reproductive traits are time consuming due to long generation interval and low heritability; however understanding the genetic factors that influence reproductive performance facilitates an accurate selection and genetic improvement of these quantitative traits. Therefore, the aim of this study was to identify the effect of lactation order and polymorphisms of FGFBP1, leptin, κ-casein, and αs1-casein genes on reproductive performance in dromedary she-camels. Blood samples were collected from one hundred she-camels using vacutainer tube containing EDTA as an anticoagulant for DNA extraction. PCR-DNA sequencing approach was performed to investigate the polymorphisms in fragments for coding sequence (CDS) parts of FGFBP1 (539-bp) and leptin (359-bp), 5′-flanking region of κ-casein (488-bp) and intervening non-coding fragment spanning from exon 4 to exon 6 (924-bp) of αs1-casein genes. Results revealed that there were single nucleotide polymorphisms (SNPs) in the investigated genes between enrolled she-camels except for leptin gene that elicited a monomorphic pattern. The identified SNPs allocated camels into different groups according to which animal harbored the explored SNPs. Multiple linear regression analysis (MLR) reveled that identified SNPs and parity order had significant effects on age at first calving (AFC), days open (DO), calving interval (CI), number of services/conception (NS/C) and milk yield traits. Determination coefficients (R2) value indicated also that variability in the phenotypic measurements of studied traits might be correlated with the identified SNPs in the reproduction related genes. This study highlights that parity order has a remarkable effect on reproductive performance. Single nucleotide polymorphisms in the investigated genes could be also used as proxy predictors and genetic markers that permit a marker assisted selection for reproductive performance traits in dromedary she-camels.
•In livestock, selection programs utilizing reproductive traits are time consuming.•Previous studies have elaborated molecular characterization of camel κ-casein, and αs1-casein genes.•There is no previous literature elicited polymorphisms of these genetic loci and their association with reproductive performance in dromedary camels.•The primer sequences for FGFBP1, leptin, κ-casein and αs1-casein genes were designed according to the PubMed published sequence of Camelus dromedarius.•The novelty of the current study is indicating the effectiveness of using MLR to test single nucleotide polymorphisms in FGFBP1, leptin, κ-casein, and αs1-casein genes as predictors for reproductive performance in dromedary camel.