Abstract
Interlinked aggregation induced emissive (AIE) fluorophores (triphenylamine (TPA) donor (D) and indanedione acceptor (A)) via non-conjugated spacers (1-3) showed concentration-dependent tunable fluorescence from blue to deep red/NIR in solution and deep red emission in the solid-state. 1-3 exhibited weak broad solid-state fluorescence covering the blue to deep red region (433 to 706 nm, Phi(F) = 2.3 to 5.3%). It is noted that the non-interlinked TPA-indanedione fluorophore was non-emissive in solution but showed strong solid-state fluorescence at 611 nm (Phi(F) = 17.7%). In contrast, 1-3 showed strong fluorescence in the solution state (Phi(F) = 0.001 to 0.09 compared to fluorescein). Importantly, 1-3 exhibited concentration-dependent tunable fluorescence from blue (457 nm) to deep red/NIR (720 nm) in CHCl3 due to varied aggregation of fluorophores. Dynamic light scattering (DLS) studies confirmed the molecular aggregation even at very low concentrations (10(-7) M). The increasing concentration lowered the solubility and produced bigger aggregates with wide size distribution that caused tunable fluorescence. The deep red fluorescence of 1 was utilized for live-cell imaging applications using E. coli cells. Fluorescence microscopic images revealed clear red fluorescent E. coli cells with a rod morphology. Furthermore, the non-toxic nature of 1 was confirmed by in vitro as well as in vivo studies. Thus, deep red/NIR emissive fluorophores were achieved by interlinking two AlEgens using a non-conjugated spacer and utilized for live cell imaging.