Abstract
Porcine gelatin has been used in many food products. To ensure the compliance of food products with halal regulations and label description, reliable analytical methods are required. In this study, Porcine-specific short amplicon-size for conventional PCB assay was developed for the detection of gelatin in commercial candy arid marshmallow products. A pair of porcine-specific primers defining a 74 by region of the mitochondria! Dnoop sequence were designed. One hundred and three samples including seventy six samples from candies and twenty seven samples from marshmallow were purchased from local market in Amman, Jordan. The results revealed that seventeen samples were found to contain porcine;gelatin ingredients and the rest 86 were found to be negative. Nine of the Positive samples were Gelatin labeled, and eight were not identified to contain gelatin. Of these samples. Five were also labeled as Halal. So, we demonstrated that the authentication of commercial sweet ingredients using PC R assay is effective and protect consumers from being mislead due to mislabels.