Abstract
Aristolochic acids (1 and 2), a component in medicinal plants, have been reported as the cause of end-stage renal failure. For the identification and quantity determination of aristolochic acid a and 2 (AA-1 and AA-2) in Radix Aucklandiae, a liquid chromatography with electrochemical detection system was constructed in this paper. Both of the chromatographic peak heights for AA-1 and AA-2 at the detection potential of -0.8 V were found to be related linearly with the concentrations ranging from 10 ng/mL to 50 μg/mL. The limit of detection (S/N = 3) of AA-1 and AA-2 are 3.7 and 3.4 ng/mL, respectively.