Abstract
Altogether, 192 Botrytis cinerea isolates collected from tomato greenhouses at different locations in Hubei Province were evaluated for their sensitivity to fungicides procymidone and zoxamide. The mean effective concentration to cause 50% growth inhibition (EC 50 ) values of procymidone for sensitive and resistant isolates were 0.25 and 3.60 mu g/ml, respectively. The frequency of procymidone-resistant (Pro(R)) isolates was 18%, and the highest frequency was recorded in Jingmen. Positive cross-resistance was observed for Pro(R) isolates to other dicarboximide fungicides but not to phenylpyrroles. Significant differences were observed for fitness parameters (i.e., mycelial growth, osmotic sensitivity, and virulence between sensitive and resistant isolates). Amino acid sequence of the Bosl gene revealed that Pro(R) isolates carried either point mutations at codon 365 (1365S) or a pair of point mutations at codons 369 (Q369P) and 373 (N373S). For zoxamide, the mean EC50 values for sensitive and resistant isolates were 0.22 and 5.32 mu g/ml, respectively. Approximately 14% of the isolates were found to be resistant to zoxamide, and the highest frequency of resistance was also observed in Jingmen. There was positive cross-resistance for zoxamide-resistant (Zox(R)) isolates to carbendazim. No significant differences were observed for fitness parameters between zoxamide-sensitive and Zox(R) isolates. Sequence analysis of the beta-tubulin gene of Botrytis cinerea revealed two previously reported point mutations (E198A and E198K) and one new point mutation (T3511). This new mutation was detected in only those isolates which possessed the E198K but not E198A substitution. This study allows for a better understanding of the resistance development profile in Hubei Province. Results will be useful for the improvement of fungicide resistance management strategies.