Abstract
Escherichia coli is an essential member of the healthy intestinal microflora of humans and different mammals. Pathogenic versions of E. coli pathotypes cause entire morbidity and mortality globally. E. coli is responsible for many outbreaks in advanced and developing countries and they every so often have deadly consequences. We used microbiological bacterium study such as gram stain, biochemical test and then used molecular diagnosed techniques the usage of ribosome gene sequence, additionally to detect toxin producing genes in some E. coli bacterium.
Our result showed that forms of bacteria had been diagnosed and distinguished between E. coli, O157: H7 and non-E. coli, O157: H7 and the usage of molecular biology method inclusive of gene collection. Some toxin-producing microorganism were recognized. Also, interpretation of the use of molecular techniques on the gene sequencing may be very useful in figuring out organisms as well as toxicity genes.