Abstract
The presence of stolbur phytoplasma in many crops all over Egypt is increasing severely. This pathogen was broadly observed in tomato leaves in a field located at Alexandria. Effective and accurate techniques depend on the PCR analysis with M1/P8 primer pair that amplifies a stolbur-specific, non-ribosomal fragments of a 720 bp. Therefore, we strongly recommend the application of DNA hybridization method for rapid and accurate detection of such economic pathogen.