Detection of pediocin PA-1 in food matrices using specific polyclonal antibodies

K. Naghmouchi; C. Lacroix; E. Kheadr; I. Fliss

doi:10.1016/j.mimet.2007.08.008

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Detection of pediocin PA-1 in food matrices using specific polyclonal antibodies

Journal article

Peer reviewed

Detection of pediocin PA-1 in food matrices using specific polyclonal antibodies

K. Naghmouchi, C. Lacroix, E. Kheadr and I. Fliss

Journal of microbiological methods, Vol.71(2), pp.175-177

01/11/2007

DOI: https://doi.org/10.1016/j.mimet.2007.08.008

PMID: 17900722

Abstract

Activity

ELISA

Fermentation

Pediocin PA-1

Polyclonal antibodies

Production

Repeated-cycle

Pediocin PA-1 was conjugated with keyhole limpet hemocyanin (KLH) and used to immunize rabbits and mice for the production of polyclonal (PAb) and monoclonal (MAb) antibodies. Titers of PAb and MAb of about 4.7 and 2.9 were obtained after three and six immunizations, respectively. An enzyme linked immunosorbent assay (ELISA) was developed for the detection and quantification of pediocin.

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Title: Detection of pediocin PA-1 in food matrices using specific polyclonal antibodies
Creators - without role: K. Naghmouchi - STELA Dairy Research Center, Nutraceuticals and Functional Foods Institute (INAF), University Laval, Quebec, PQ, Canada, G1K 7P4.
C. Lacroix - ETH Zurich
E. Kheadr - STELA Dairy Research Center, Nutraceuticals and Functional Foods Institute (INAF), University Laval, Quebec, PQ, Canada, G1K 7P4.
I. Fliss - STELA Dairy Research Center, Nutraceuticals and Functional Foods Institute (INAF), University Laval, Quebec, PQ, Canada, G1K 7P4.
Publication Details: Journal of microbiological methods, Vol.71(2), pp.175-177
Publisher: Elsevier B.V
Identifiers: 9912100108331
Academic Unit: Al Baha University
Language: English
Resource Type: Journal article