Abstract
In this study, a novel
in situ
choline sensor was assembled by attaching the binary Mn
2
O
3
/NiO nanoparticles (NPs) onto a glassy carbon electrode (GCE). Initially, Mn
2
O
3
/NiO NPs were synthesized
via
a wet-chemical process and fully characterized
via
XRD, XPS, FESEM, EDS, FTIR and UV-Vis methods. The analytical performances of the choline sensor were evaluated by an electrochemical method in the phosphate buffer phase. The estimated linear dynamic range (LDR) was found to be 0.1 nM to 0.1 mM. The other analytical performances of the choline sensor, such as sensitivity (16.4557 μA μM
−1
cm
−2
) and detection limit (5.77 ± 0.29 pM), were also calculated very carefully from the calibration plot. Overall, the choline sensor exhibited a reliable reproducibility,
in situ
validity, selectivity, interference effect, stability, and intra-day and inter-day performances with high accuracy in a short response time. Moreover, the probe was successfully applied to detect choline in real human, mouse and rabbit serum. This fabrication route would be a novel approach for the detection of selective biochemical sensor in the healthcare and biomedical fields.