Abstract
Encarsia bimaculata was recently described from India as a potentially useful parasitoid of
Bemisia tabaci. Its developmental biology was studied in the laboratory at 25–30
°C and 70–75% RH. Results showed that
E. bimaculata is a solitary, arrhenotokous, heteronomous, autoparasitoid. Mated females laid eggs internally in
B. tabaci nymphs that developed as primary parasitoids. Males developed as hyperparasitoids, either in females of their own species or in other primary aphelinid parasitoids. Superparasitism was common under cage conditions. Both sexes have an egg, three larval instars, prepupal, and pupal stages. Development from egg to adult took 12.70
±
2.10 days for females and 14.48
±
2.60 days for males. Individual
B. tabaci nymphs were examined for
E. bimaculata parasitization using three isozymes: esterase, malate dehydrogenase, and xanthine dehydrogenase. All three isozymes showed differential banding patterns that identified
E. bimaculata parasitized or unparasitized
B. tabaci nymphs.