Abstract
Dietary arginine requirement of Heteropneustes fossilis fry (3.0 +/- 0.5 cm; 5.1 +/- 0.3 g) was determined by feeding casein-gelatin-based isonitrogenous (400 g kg(-1) crude protein) and isocaloric (17.97 kJ g(-1)) amino acid test diets containing graded levels of L-arginine (15, 17, 19, 21, 23 and 25 g kg(-1) dry diet) for 12 weeks. Maximum absolute weight gain (AWG) (44.4), best feed conversion ratio (FCR) (1.22), highest protein retention efficiency (PRE%) (41%), energy retention efficiency (ERE%) (75%), best condition factor, hepatosomatic index and viscerosomatic index were noted at 21 g kg(-1) arginine of the dry diet. Maximum body protein (189.8 g kg(-1)) was also obtained in fish fed above diet. Highest haematocrit value (35%), Hb concentration (9.54 g dL(-1)), RBC count (3.44 x 10(9) mL(-1)) and lowest Erythrocyte sedimentation rate (ESR) (1.93 mm h(-1)) were obtained at the above level of arginine in the diet. AWG, FCR, PRE% and ERE% data were analysed using broken-line and an exponential fit to obtain more precise dietary arginine requirement. On the basis of broken-line and exponential analyses of AWG, FCR, PRE and ERE data, inclusion of dietary arginine in the range of 20.4-22.6 g kg(-1) dry diet, corresponding to 51-56.5 g kg(-1) dietary protein, is recommended for formulating arginine-balanced feeds for rearing H. fossilis fry.