Abstract
To gain insight into the differential mechanism of gene promoter hypermethylation in acute and chronic leukemia, we identified the methylation status on one part of 5'CpG rich region of 8 genes,
DAB2IP
,
DLC-1
,
H-cadherin
,
ID4
,
Integrin α4
,
RUNX3
,
SFRP1
, and
SHP1
in bone marrows from acute myeloid leukemia (AML) and chronic myeloid leukemia (CML) patients. Also, we compared the methylation status of genes in AML and CML using methylation-specific PCR (MSP). The frequencies of DNA methylation of
ID4
,
SFRP1
, and
SHP1
were higher in AML patients compared to those in CML patients. In contrast, no statistical difference between AML and CML was detected for other genes such as
DLC-1
,
DAB2IP
,
H-cadherin
,
Integrin α4
, and
RUNX3
. Taken together, these results suggest that these methylation-controlled genes may have different roles in AML and CML, and thus, may act as a biological marker of AML.