Abstract
The present study was conducted to evaluate the potential effect of hesperidin on the regulating Th1, Th2, Th17, and the proinflammatory cytokines production from splenocyte of acute murine schistosomiasis. Each mouse was infected with 100 cercariae of Schistosoma (S.) mansoni and at 8 weeks post-infection, mice were sacrificed, splenocytes were prepared and cultured in the presence of either soluble adult worm antigen (SWAP) or soluble egg antigen (SEA) of S. mansoni with either 50 or 100 mu M hesperidin. Hesperidin, in both tested concentrations, significantly augmented interleukin (IL) 4 (p < 0.05), IL-10 (p < 0.01), and interferon gamma (IFN-gamma) (p < 0.05) production in response to SAWP and SEA stimulation. However, IL-13, IL-17, IL-1, and tumor necrosis factor alpha (TNF-alpha) production were significantly (p < 0.01) reduced. Interleukin 12 production was not significantly (p > 0.05) changed with hesperidin treatment at 50 mu M, while 100 mu M concentration of hesperidin significantly reduced IL-12 production in response to SWAP (p < 0.05) and SEA (p < 0.01) stimulation. The results suggested that hesperidin preferentially modulated in vitro production of Th1, Th2, Th17, and proinflammatory cytokines and this could reduce immunopathology of schistosomiasis.