Abstract
► Rapid up-regulation of intestinal and kidney GR1, GR2 and MR transcripts on day 1 may increase tilapia SW tolerance. ► The GR2 and MR have distinctive expression patterns in gill on day 1 and 4, respectively to increase the SW tolerance. ► The coordinated action of GR1, GR2 and MR provides a new perspective of regulation during SW acclimation in tilapia.
The glucocorticoid receptor (GR) plays an essential role during seawater (SW) acclimation. However, the regulation of GR isoforms 1 and 2 (GR1 and GR2) and the mineralocorticoid receptor (MR) during SW acclimation is poorly understood. To address this, we localized and examined the GR1, GR2 and MR transcripts in the tilapia gill, kidney and intestine. Our results indicated that the GR1, GR2 and MR levels were increased in the kidney and intestine on day 1 in seawater (SW) fish, which is in agreement with the recognized osmoregulatory role of the corticosteroid receptors. The SW transfer increased the GR2 and MR transcripts in the gill on day 1 and 4, respectively. Surprisingly, no significant difference was obtained for the GR1 mRNA level. Analysis of the plasma parameters in freshwater (FW) and SW tilapia showed that the plasma cortisol levels were significantly increased at day 1 in the SW fish compared to the FW fish. This is the first study that focused on the spatial distribution of GR1, GR2 and MR in the osmoregulatory organs of freshwater (FW)- and SW-acclimated tilapia by in situ hybridization. Consistent with the Q-PCR results, the expression levels of the GR1, GR2 and MR transcripts were increased or decreased in the SW-acclimated tilapia’s gill, kidney and intestine compared to the FW fish. We observed that GR1, GR2 and MR were localized in the branchial epithelial cells and chloride cells of the gill, proximal tubules of the kidney and columnar cells of the intestine. Together, these results indicate that the mobilization of corticosteroid receptors is dependent on the target tissue, salinity and exposure time.