Abstract
Objective: To identify staphylococcal microorganism isolated from dental clinics, detect the impact of some disinfectants on removing the preformed biofilms and to demonstrate the adhesion of cells on the surfaces of some materials used for manufacturing the dental material using Scanning electron microscope (SEM). Material and methods: Out of seventy totally different swabs, twelve staphylococcal isolates were recovered. All isolates were tested for susceptibility to thirteen antimicrobials. The isolates were screened for biofilm production using microtiter plate (MtP) test, moreover the efficacy of some disinfectant as removal the agents of preformed biofilm. Scanning electron microscope (SEM) was used for demonstrating biofilm formation by selected isolates on stainless-steel and rubber surfaces. Results: Of twelve staphylococci isolates, 33.3, 16.6 and 50 % were strong, moderate and weak biofilm producers, respectively. Adhesions of the preformed biofilm were found to be reduced within the presence of Betadine and sodium bicarbonate. SEM images showed the capability of biofilm formation by S. aureus H3 and S. hominis M0401 on stainless-steel and rubber surfaces. Conclusion: We conclude that establishing effective preventive ways for well-practiced infection management is crucial to prevent staphylococcal infections and promote a secure surroundings within the dental clinics by eradicating biofilms formed on medical devices. These substances may be betadine and sodium bicarbonate.