Abstract
Delivering accurate radiation dose to blood specimens during biological irradiations is essential in quantifying damage of ionizing radiation. To estimate dose to blood samples as accurately as possible, pieces of EBT2 model GAFCHROMIC™ film were placed within an approximately 10 mm finely ground rice layer that was used to simulate test specimens inside 40 mL plastic flasks. Irradiations of flasks were carried out using an X-RAD 320 irradiator with a beam quality of 320 kVp and a measured half value layer of 1.12 mm Cu, in air and in a full scattering setup which consisted of either rice or Solid Water™ (SW) surrounding flasks, filled to the same level at top of the flasks, together with a 5 cm thick SW slab beneath them. Outputs, per cent depth doses and beam profiles at different depths were measured and compared between setups. For the same setting, the dose delivered to the middle flask under the full scattering setup is 22% larger than with the in-air setup at the depth of the specimen and 9.2% more homogeneous across the specimen thickness of 10 mm (2.3% variation in comparison to the surface). Rice showed a fairly similar performance to SW within 1% at the same depth of 10 mm. Experimental setup based on full scattering conditions was shown to provide faster, more homogenous and fairly uniform dose delivery to biological specimens in comparison to conventionally used in-air setups.