Abstract
Twenty seven (
) known natural organic compounds were isolated for first time from two species of
, i.e.
and
The structures of these compounds were deduced from the spectral data of NMR, IR, and mass spectrogram. These were evaluated against urease and carbonic anhydrase inhibition studies. For carbonic anhydrase-II inhibition studies, these compounds were evaluated by biochemical mechanism based
bio-assay. Some compounds showed significant inhibition against CA-II enzyme. Compartively, compound (
) showed IC
value of 17.60 ± 0.08 μM against urease enzyme, while compound (
) was found to be most active against carbonic anhydrase-II, having an IC
value of 66.27 ± 0.89 μM. Izalpinin (
), 5,7-dihydroxy-2′,6-dimethoxyisoflavone (
), 4′,5,7-trihydroxy-6-methoxyflavanone (
), 4′,5,7-trihydroxy-3′,8-dimethoxyflavanone (
), 8-methoxyeriodictyol (
), and mangiferin (
) were found to be dual inhibitors of both the enyzmes. The most active compounds were docked using Autodock Vina and i-GEMDOCK softwares. The docking and
results are in agreement which showed secondary interactions with the enzymes. The compounds can serve as therapeutic agents to treat urease and carbonic anhydrase associated disorders.