Abstract
The stems, leaves and fruiting bodies of L. siceraria is considered a renewable natural resource of a several biological active compounds. The aim of present study was extraction of a novel antibiotic agent inhibit to some antibiotics resistant clinical microorganism. The fruit bodies of L. siceraria collected from local market in Riyadh city, Saudi Arabia. The dried fruiting bodies was extracted with n-hexane, chloroform, methanol in a sequential manner. The biological active test showed that the crud chloroform extract was the best among other crud extracts, so it fractionated by silica gel column using chloroform (15): Methanol (1): n-hexane (16). The purity and biological test were performed to choice the pure, active fraction for following studies. The susceptibility test and infrared analysis were carried out. The results indicated that fraction number 3 (NF-3), was good inhibitor against antibiotic resistant bacteria (Bacillus cereus, Pseudomonas vulgaris, Klebsiella pneumonia, Streptococcus mutans and Escherichia coli.) and Candida albican whereas had not any activity against Salmonella typhimurium and Aspegillus niger. NF-3 was semi-pure compound containing a many of functional groups such as N=O, C-O, C=O ester, ketone and C-H aldehyde. The data suggested that the fruiting bodies of Lagenaria cineraria (Molina) are considered a good renewable natural resource for antimicrobial agents.