Abstract
A novel reverse phase high performance thin layer chromatography (RP-HPTLC) method has been established for the estimation of valerenic acid (VA) in methanolic extract of Valeriana officinalis and commercial products using green solvent. RP-HPTLC analysis was carried out using ethanol-water (9:1 v/v) as the mobile phase. The densitometric analysis of VA was performed at 226 nm. The chromatographic peaks of VA from plant extract of V. officinalis and marketed herbal products were found to be identical with a single spot at Rf = 050 +/- 0.02. Linear regression analysis revealed a good linear relationship between the peak area and amount of VA. Linearity range was recorded as 500-6000 ng/band. The validation was tested in accordance with ICH guidelines for precision, accuracy and robustness. The proposed eco-friendly RP-HPTLC method was found to be suitable for the analysis of the therapeutic dose of VA in methanolic extract of V. officinalis and commercial products.