Abstract
The present study aimed to investigate the effects of silage quality, microbial contents and fermentation metabolites of mixed ratio of barley and pea with the supplementation of chlorella with Lactobacillus plantarum under field conditions of livestock farmers and to monitor the responses of silage variables after forty five days. After silage preparation completed, the contents of dry matter (DM), crude protein. (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF), RFV (Relative feed value), crude ash (CA) and microbes such as lactic acid bacteria (LAB), yeast and fungi counts, and fermentation metabolites such as lactic acid, acetic acid and butyric acids were analyzed. Results indicated that the nutritive profiles were significantly changed with respect to the concentration of chlorella. Addition of L. plantarum further improved the silage quality. Among the microbes, LAB showed dominant and counts were recorded in barley and pea silages (6.92 and 6.99 x 10(7) cfu g(-1)) and 50 % barley with 50 % pea and 80 % barley with 20 % pea (6.869 and 4.12 x 10(7) cfu g(-1)) respectively, whereas the average numbers of yeast and fungi significantly less. The pH of the samples was ranged from 3.6-5.45. Lactic acid detected as the dominant organic acids, detected higher amount (14.59%) in 0.25% chlorella supplemented pea silage. This result confirmed that silage preparation using different crops with the supplementation of chlorella and L. plantarum inoculation is most beneficial for farmers.