Abstract
CARBONIC anhydrase catalyses hydration of aldehydes1-3:
I have studied the relevance of hydration of glyceraldehyde-3-phosphate (GAP) in its oxidation by GAP dehydrogenase. GAP dehydrogenase activity was assayed spectrophotometrically by measuring the increase in absorbance at 340 nM of a reaction mixture containing 0.1 M Tris-HCl (pH 8.5), 17 mM sodium arsenate, 5 mM cysteine, 20 mM NaF, 1 mM NAD, 0.1 U ml. of GAP dehydrogenase (Sigma) and 4 µM GAP.