Abstract
The present study aims to illustrate the effect of P. aeruginosa (ATCC9027) to promote the biofilm formation on the modified Ni-Cu-P alloy coating supported on Aramco carbon steel C1018. The microbial biofilm formation of P. aeruginosa on C1018 and Ni-Cu-P alloy in artificial seawater and seawater containing crude oil was investegated. Scanning electron microscope (SEM), and energy dispersive X-ray surface analysis techniques were used to confirm the morphology and chemical composition of the alloy coating. On the other hand, the colony forming units of P. aeruginosa on carbon steel C1018, alloy coating and Heat treated Ni-Cu-P alloy coating was examined by the plate-counting method and the construction of the biofilm was distinguished by SEM analysis. Heat treated Ni-Cu-P alloy coating record the highest antibacterial efficiency via the formation of the thick biofilm layer. The produced modified alloy coating with the thick, spongy like biofilm layer are a high-quality protective cover for carbon steel to enhance its efficiency for petroleum industrial applications.