Abstract
The fate and brain targeting effects of ropinirole loaded true nanoemulsion (NEROP) by intranasal administration were assessed and the effects of homogenization was evaluated for any further uptake by olfactory nerves. Formulations (true and homogenized nanoemulsion) were characterized for drug content, percentage transmittance, globule size and zeta potential. The nanosizing potential on brain bioavailability was examined with the help of gamma scintigraphy using dynamic model. Two different radiolabelled nanoformulations as Tc-99m-NEROP and Tc-99m-Susp(ROP) were intranasally administered in experimental animals with the dose level of 100 mu L 2.5 kg(-1). The brain translocalization of optimized nanotherapeutics via olfactory nerve were assessed by continuous imaging of sixty frames programmed in gamma camera. The images obtained in sixty frames were used to calculate the radio-cunts in the specified brain and bottom region. For brain pharmacokinetic measurement, a graph was plotted between time lapsed and radio-counts. A significant difference in brain localization and C-max were observed between (TC)-T-99m-NEROP and Tc-99m-Susp(ROP) formulations. These results showed that formulation must be directly transported from the nasal cavity into the CSF. Finally it was concluded that, the homogenization effect drastically improved brain uptake of ropinirole.