Abstract
The individual and combined effects of micronutrients and hormones on freshwater-borne microalgae growth were investigated for biofuel feedstock in this experimental study. Five algal strains of Chlorella sp., Chlorococcum sp., Phormidium sp., Chlorella sp. and Cosmarium sp., AZH, AZS, ZAA1, ZAA2, and ZAA3, respectively, have been investigated. These strains were treated using different concentrations of micronutrients (iron chloride, manganese chloride, and sodium molybdenum oxide) and hormone (salicylic acid). The different treatments' growth effects were as follows: iron chloride > sodium molybdenum oxide > manganese chloride > salicylic acid. The order of the increases in the number of microalgal strain cells achieved by the application of the micronutrients and hormone was AZH > AZS > ZAA3 > ZAA2 > ZAA1. The combined treatments produced higher growth rates than the individual treatments, with the order of their effects being micronutrients + hormone > all three micronutrients > hormone alone. The increase in the number of microalgal strain cells under combined treatment was ZAA3 > AZH > AZS > ZAA2 and assessed using one-way ANOVA.