Abstract
Herpes simplex virus type 1 (HSV-1) infection is a health concern worldwide, it is responsible for the orolabial lesions, ulceration and several mild and severe diseases. The current study was carried out to investigate the in vitro antiviral activity of Punica granatum, Pomegranate peel extracts (PPE) and Lepidium sativum (Garden Cress) seed extract (GCSE) on Herpes Simplex Virus Type 1. The IC50 values for PEE C4H10O and CHCl3 were 23.72 mu g/ml, 191.71 mu g/ml respectively whilst the IC50 for GCSE was 53.37 mu g/ml.
The antiviral activity of each extract was estimated using end point assay and infectivity titer was calculated according to Reed and Muench equation. Data recorded revealed that HSV-1 seed stock was 10(8)/ml, The IC50 values of tested extracts were 23.72 mu g/ml, 53.37 mu g/ml and 191.71 mu g/ml for C4H10O, GCSE and CHCl3 respectively.
The antiviral assay showed variable potentials relative to the test extract as the infectivity titer reduction arranged in the order of Diethyl ether (C4H10O) recording 1.5 log (10) /ml (21.4%), Chloroform (CHCl3) recording 0.67 log (10) /ml (9.7%) and GCSE recording 0.25 log (10) /ml (3.6 %). taken together.