Abstract
Simple Summary The present study shed light on the effect of salinity on the plant growth and secondary metabolites of medicinally important milk thistle plant ecotypes. At the same time, we also studied the effect of external supplementation with ascorbic acid, thiourea, and moringa leaf extract on improving growth-related attributes and secondary metabolites under salinity stress. Various parameters were studied related to stress alleviation. Ascorbic acid, followed by moringa leaf extract, was the most effective in improving growth under salt stress conditions. The present study demonstrated that milk thistle could withstand moderate doses of salt stress, while externally supplemented media improved all the growth parameters by increasing the accumulation of secondary metabolites. Milk thistle (Silybum marianum (L.)) is a wild medicinal herbal plant that is widely used in folk medicine due to its high content of secondary metabolites (SMs) and silymarin; however, the data regarding the response of milk thistle to salinity are still scarce and scanty. The present study evaluated the effect of salinity on a geographically diverse population of milk thistle and on the role of medium supplementation (MS) with ascorbic acid, thiourea, and moringa leaf extract in improving the SMs and growth-related attributes under salinity stress (SS). For germination, a 120 mM level of salinity was applied in the soil during the seedling stage. After salinity development, predetermined levels of the following compounds were used for MS: thiourea (250 mu M), moringa leaf extract (3%), and ascorbic acid (500 mu M). The data regarding growth attributes showed that SS impaired plant growth and development and increased SM production, including alkaloids, anthocyanin, and saponins. Moreover, ascorbic acid, followed by moringa leaf extract, was the most effective in improving growth by virtue of increased SMs, especially under salt stress conditions. The present study demonstrated that milk thistle could withstand moderate doses of SS, while MS improved all the growth parameters by increasing the accumulation of SMs.