Abstract
Objective: To determine the effect of high dose thiamine on dyslipidemia in diabetic rats.
Study Design: Experimental interventional study.
Place and Duration of Study: The Animal House/Laboratory of the National Reference Laboratory for Poultry Diseases (NRLPD)/Biochemistry Department, Army Medical College, Rawalpindi, from December 2006 to January 2007.
Methodology: The study was conducted on 120, 12 weeks old male albino rats of Sprague Dawley Strains randomly divided into 4 groups of 30 rats each. Group I comprised of 30 normal rats, on normal (regular) diet. Group 11 comprised of 30 diabetic rats, on normal (regular) diet. Group III comprised of 30 normal rats, on thiamine supplemented diet. Group IV comprised of 30 diabetic rats, on thiamine supplemented diet. Out of the 120 rats, 60 were made diabetic by injecting Alloxan. Blood glucose levels were measured by applying glucose oxidase method. Determination of total HbA1c, triglyceride, HDL (High Density Lipoproteins) and total cholesterol was performed by diagnostic kits. The significance of difference in mean values of study groups (glycemia and lipid profile) was assessed by applying ANOVA and independent Student's t-test.
Results: Significant difference was found in the levels of triglycerides, cholesterol and LDL in the diabetic rats on supplemented diet p <0.05, but no significant difference was noticed in the value of glycosylated Hb (HbA1c) (p>0.05) of all the 4 groups.
Conclusion: Thiamine corrected dyslipidemia but not hyperglycemia in diabetic rats.