Abstract
Threonine (Thr) may be a limiting amino acid for laying hens fed diets with lowered protein level. An experiment was conducted to examine laying performance, and the intestinal immune function of laying hens provided diets varying in digestible Thr levels. Lohmann Brown laying hens (n=480), 28weeks of age, were allocated to six dietary treatments, each of which included five replicates of 16 hens. Dietary crude protein (CP) 16.18% diet was offered as the positive control diet. L-Thr was added to the negative diet (14.16% CP) by 0, 1.0, 2.0, 3.0 and 4.0g/kg, corresponding 0.44%, 0.43%, 0.49%, 0.57%, 0.66% and 0.74% digestible Thr. At 40weeks, a reduction in CP level decreased laying performance (p<0.05). In the low CP, increasing dietary Thr increased (p<0.05) egg production and egg mass and rose to a plateau between 0.57% and 0.66%. The hens fed 0.66% Thr showed the lowest value (p<0.05) of feed conversion ratio (FCR). Serum level of uric acid showed the lowest values (p<0.05) at 0.57-0.66%. In addition, serum-free Thr maximized (p<0.05) between 0.66% and 0.74%. Digestive trypsin activity decreased (p<0.05) when hens fed the low-CP diet compared with hens fed CP (16.18%) and hens fed 0.57-0.66%. Expressions of ileal MUC2 mRNA maximized (p<0.05) at 0.66% Thr. Occludin mRNA increased with increasing Thr level (p<0.05). sIgA mRNA reached to the maximum level (p<0.05) at 0.66% and 0.74% Thr. INF- mRNA reached to the lowest level (p<0.05) at 0.65%. Expressions of ileal IL-2, IL-6, IL-1 mRNA decreased with increasing Thr level (p<0.05). In conclusion, Thr supplementation resulting in optimal laying performance and stimulated the mucosal immune system, suggesting that it is a limiting amino acid in the low-crude-protein diet of laying hens during the peak production period.