Abstract
A total of 640 layer hens, 56 wk of age, were allocated to 4 dietary treatments groups, each of which included 5 replicates of 32 hens. Graded levels of L-Thr were added to the basal diet at 0.0 (control), 1.0, 2.0, and 3.0 g/kg; determined Thr values were 5.7, 6.5, 7.4, and 8.7 g/kg of diet, respectively. In the 53-d feeding trial, egg production increased significantly due to dietary L-Thr supplementation. Hens fed 2.0 g of Thr/kg of diet had significantly higher egg production than the control diet. Supplementing the diet with L-Thr did not affect egg weight or feed intake, but egg mass increased significantly. Also, FCR was improved significantly and the best FCR was exhibited by hens receiving 2.0 g of Thr/kg of diet. Feeding laying hens a diet containing 2.0 g of L-Thr/kg of diet improved Haugh units linearly; however, egg shell thickness decreased linearly as the level of dietary Thr increased, and the lowest value was observed for hens fed 3.0 g of Thr/kg of diet. Serum free Thr increased linearly as supplemental Thr increased, but serum free Ile, Phe, and Tyr decreased. No differences attributable to L-Thr were found among groups in the activity of digestive enzymes (trypsin and amylase) in the mucosal homogenates of the jejunum. The activity of alpha-alkaline phosphatase in the jejunum was analyzed as a marker of mucosal enterocyte maturation and no differences due to L-Thr supplementation were noted. In conclusion, feeding laying hens from 56 to 64 wk of age L-Thr up to 2.0 g/kg can improve laying performance and Haugh units.