Abstract
An immensely useful technique was established to elucidate suitable culture conditions for in vitro micropropagation of Calanthe hybrids by using modified Hyponex media. Among the light emitting diodes (LEDs), the mixture of blue plus red light efficiently enhanced in vitro growth of the plantlets, whereas inhibitory effect on the plantlets growth was observed under the mixture of red plus far-red light. However, the effect of light quality on the growth of plantlets was more pronounced in 'Bukduseong' x 'Hyesung' hybrid compared to 'Chunkwang' x 'Hyesung'. Of the various gradients of sucrose tested (0, 7.5, 15, 30 and 60 g l(-1)), 15 g l(-1) sucrose was proven suitable concentration for enhancing growth and growth attributes of 'Bukduseong' x 'Hyesung', while 15 and 30 g l(-1) sucrose were regarded as an optimal concentration for in vitro growth of the plantlets of 'Chunkwang' x 'Hyesung' hybrid. Although, higher sucrose concentration (60 g l(-1)) enhanced root growth but induced plantlets abnormality. In addition, as a source of natural product, 50 ml l(-1) coconut water effectively enhanced plantlets growth of both hybrids compared to the relative control (without coconut water). After 8 weeks of culture, when the plantlets were transferred to the green house, 85% survivability of the plantlets was achieved upon hardening.