Abstract
The present study was aimed to isolate active constituents fromBlumea axillaris(Lam.) DC (Asteraceae) against phytopathogenic fungi. Bioactivity guided fractionation of the successiven-hexane, chloroform and methanol extract led to the isolation of the monoterpene ester (4 R,5S)-4-hydroxy-7-tigloyloxycarvotanacetone (1). The compound 1 was converted into acetyl derivative (2). The acetyl derivative (2) and the parent compound 1 were tested again phytopathogenic fungi by using mycelial inhibition and minimal inhibitory concentration values were found out by the broth microdilution method. The acetyl derivative (2) showed the highest antifungal activity againstRhizoctonia solaniandAspergillus niger. Based uponin vitroresults, compound 1 was tested againstFusarium oxyporum(wilting disease) and compound 2 was tested againstR. solani(leaf blight disease)in vivousing the foliar spray method. Both compounds had no phytotoxicity and alsoin silicodocking study showed that both compounds were binding similarly as commercial fungicide carbendazim.