Abstract
Objective: To evaluate the influence of hemostatic agents (Expasyl and Viscostat) on the bond values of RMGIC bonded to pretreated dentin surface with cavity conditioner (CC), self etch primer (SEP), and antimicrobial photodynamic therapy (aPDT) Materials and methods: One hundred and eighty human third molars were embedded in a self-cure acrylic resin filled polyvinyl cylinder. The dentinal surface was prepared and all the samples were arbitrarily distributed into three different groups liable to the hemostatic agent used (n = 60). Samples in group 1: no hemostatic agent used, Group 2: Samples exposed to Expasyl, and group 3: Viscostat. After applying the hemostatic agent, all the samples were surface treated with different surface conditioners. Subgroup A1, B1, C1 were conditioned with aPDT, Subgroup A2, B2, and C2 surface were treated with SEP, and Subgroup A3, B3, and C3 were smeared with CC. RMGIC was placed and SBS was assessed using a universal testing machine and failure mode analysis was performed using and stereomicroscope at 40x magnification. Analysis of variance (ANOVA) and Tukey's post hoc test compared the means and standard deviations (SD) following SBS testing (p = 0.05). Results: Highest SBS was exhibited by group A2 (22.36 +/- 1.66 MPa Control SEP) whereas group A1 (14.14 +/- 1.32 aPDT + Control) demonstrated the lowest SBS. Subgroups B1 (17.23 +/- 0.33-Expasyl + aPDT) and C1 (18.36 +/- 0.25 MPa-Viscostat + aPDT) were found to be comparable (p > 0.05). Similarly, A3 (19.69 +/- 1.01 Control + CC) and B3 (18.23 +/- 0.33 MPa-Expasyl + CC) and C3 (18.36 +/- 0.37 Viscostat + CC) demonstrated no significant difference in bond scores (p > 0.05). Most of the failures in aPDT were adhesive. However, SEP and CC groups demonstrated admixed and cohesive failure Conclusion: Dentin conditioned by aPDT after hemostatic agents improves bond values of RMGIC. Self etch Primer and Cavity conditioner using polyacrylic acid without hemostatic agent improves bond integrity of RMGIC.