Abstract
Chlortetracycline (CTC) degradation using potential microbial consortia or individual bacterial strains was useful method for improving bioremediation potential. The co-culture (Klebsiella pneumoniae CH3 and Bacillus amyloliquefaciens CS1) of bacterial strains have the ability to degrade chlortetracycline (91.8 +/- 1.7%), followed by sulfamethoxazole (62.1 +/- 1.2%) and amoxicillin (73.9 +/- 3.3%). It was observed that the degradation potential was maximum after 10 days incubation, 8-10% inoculum, pH 7.5, and antibiotic concentration ranged from 150 to 200 mg/L. The initial concentrations of CTC significantly affected CTC degradation. In strain CH3, maximum biodegradation of CTC (99.4 +/- 2.3%) was observed at 200 mg/L initial CTC concentrations. In CS1, maximum biodegradation of CTC was obtained at 150 mg/L concentration (80.5 +/- 3.2%) after 10 days of culture. Alkaline pH was found to be suitable for the degradation of antibiotic than acidic range. After initial optimization by one factor at a time approach in free cells, the bacterial strains (CH3 and CS1) were co-immobilized. The co-immobilized bacterial cells showed improved degradation potential than free cells. To determine the biodegradation potential of immobilized cells, the selected strains were immobilized in polymer beads and treated with CTC with 175 mg/L initial concentration. The experimental results revealed that after 3 days of treatment the residual CTC concentration was 150.1 +/- 3.2 mg/L and it decreased as 1.28 +/- 0.01 mg/L after 10 days of treatment. The present study confirmed the effectiveness and feasibility of biodegradation ability of K. pneumoniae CH3 and B. amyloliquefaciens CS1 immobilized for CTC degradation in wastewater.