Abstract
Background and objectives
Although there are several toxicological studies on aspartame (ASP), its histopathological effects on the liver have received little attention. Natural marjoram [Origanum majorana (OM)] is extensively studied for its ability to protect cells from damages. The present study was to evaluate the chronic effects of ASP, and marjoram as well as the protective effect of aqueous extract of marjoram leaves (OM) against ASP-induced liver toxicity.
Materials and methods
Seventy-two female albino rats (180 +/- 20g) were divided into nine groups: G1 as a control, G2 and G3 received ASP, G4 and G5 were administrated water extract OM, and G6, G7, G8, and G9 were treated with ASP+OM. Serum glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, vascular endothelial growth factor levels, and histological examination of the liver tissues were detected for all groups.
Results and conclusion
ASP caused a significant increase in glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, and vascular endothelial growth factor levels. A combination of OM (100 or 300 mg/rat) with ASP (50 or 100 mg/rat) improved most of the previous parameters. Histological examination of the liver showed some abnormality, but no significant morphological changes in G6, G7, G8, and G9. The treated rats (G2 and G3) showed hepatic fibrosis, nuclear changes, hepatocyte degeneration disruption hepatic cords, and cytoplasmic vacillations. ASP groups treated with OM showed improvement of many hepatic changes. Thus, OM may have a protective effect on ASP hepatotoxicity.