Abstract
Follicular fluid (FF) is one of the important sources of micro-organisms that may affect IVF outcomes. IVF cultures were found to contaminate the fungi. The source of the contaminating fungus is possibly from the FF, and the culture medium does not regularly contain any antifungal agents, which is the cause of widespread fungal contamination in the culture of IVF. A lot of people are therefore opting for IVF to get a child. In vitro fertilization, assisted reproductive technologies (ART) includes extracorporeal fertilization using specific surgical procedures to support pregnant people. It is typically carried out when certain, less costly forms of reproduction struggle. Infertility is a global public health concern and accepted by Saudi society as a big issue. Many triggers can contribute to female infertility, such as ovulatory disorders, endometriosis, endocrine disorders, genetic factors, tubal factors, and pelvic inflammatory disease. Additionally, many factors in lifestyle, such as age, weight, obesity, smoking, environmental and other toxins, can affect overall health and lead to infertility. The embryo culture medium also contains antibacterial agents, but some species of bacteria may be resistant to these antibiotics and do not routinely produce any antifungal agents. The FF was not always sterile but contained a range of microorganisms that affected IVF results, and a broader sample of patients needed to be studied to further confirm our theory. Furthermore, identification of FF microbes in women with repeated failed IVF cycles will provide an opportunity to start antimicrobial therapy before the next pregnancy. The study aimed to provide a summary of the microorganisms and their effects on in vitro fertilization of human follicular fluid..