Abstract
Present study aimed to investigate the effect of adding antioxidants, cysteine and ascorbic acid on the levels of glutamic oxaloacetic transaminase (GOT), glutamic‐pyruvate (GPT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and γ‐glutamyl transpeptidase (GGT) enzymes of post‐thawed stallion sperm. Ten ejaculates were collected each from four healthy stallions and cryopreserved using HF‐20 freezing extender containing either 0 mg/ml cysteine or ascorbic acid, 0.5 mg/ml cysteine and 0.5 mg/ml ascorbic acid. All samples in freezing extender containing cysteine or ascorbic acid or none of them were assessed for sperm motility, viability, plasma membrane integrity, morphology and enzymes concentration. The ALP, LDH and GGT were significantly higher in 0‐group compared with cysteine and ascorbic acid groups. The sperm motility of frozen‐thawed semen with 0‐group was significantly better compared with cysteine and ascorbic acid groups. The variation on viability, sperm membrane integrity and morphology were insignificant between all treated groups. Therefore, these enzymes were reduced when using antioxidants in the freezing extender. Results of the present study suggest that concentration of ALP, LDH and GGT enzymes could be used as parameters for prediction of frozen‐thawed stallion semen.
This manuscript aims to evaluate the effect of cysteine and ascorbic acid to HF‐20 extender on the frozen semen enzyme and using these enzymes as markers of frozen semen quality in stallion's semen. It shows that the level of ALP, LDH and GGT enzyme steadily correlated with post‐thawed semen quality. The samples treated with cysteine and ascorbic acid showed a deleterious effect of sperm enzyme concentration and function compared with control group.